![]() ![]() 2007) whose genomic information in the public domain is very limited however, is predominately used as traditional medicine and has increasingly received attention in terms of harvestable biochemical constituent properties (Tshivhandekano et al. Monsonia burkeana is one of the members of the genus Monsonia (Touloumenidou et al. In the current study, we embarked on de novo sequence assembly and profiling of a partial transcriptome for the tea plant, Monsonia ( Monsonia burkeana Planch. Identification of plant products that have values for human health using the transcriptome assembly is currently widely applicable both in the reference-guided and de novo fragments particularly in non-model plant species whose genome sequence is as yet unavailable or incomplete. Recent advances in techniques used in studies on gene expression and genome mapping evolved to converge on RNA sequencing as a comprehensive and efficient way to measure transcriptome composition, obtain RNA expression patterns, and discover new genes and gene features and accurate transcriptome quantification for single cells (Morozova et al. Sequencing the transcriptome is a fast, cost-effective and viable alternative to sequencing the genome and provides a snapshot of expressed genes. Thus, DNA sequencing and genome assembly, and RNA sequencing and transcriptome assembly remain to be ongoing projects as a corner stone for comprehensive sequence-based analysis that enables addressing of novel questions in most areas of biological and agricultural research. Today, we are able to witness these rapidly evolving novel technologies that require automated protocols for generating sequencing libraries with effective and different approaches to genomic sequence analysis and to experimental designs. The emergence of next generation sequencing (NGS) has provided immense practical advantages for comprehensive analysis of sequences at the genome and transcriptome level, through widely accessible massively parallel short-reads sequencing platforms. burkeana leaf transcriptome gives insight into tea and drug-specific products, therefore representing basis in further investigation of the plant. The relatedness of more than 80 gene families encoding key enzymes was shown using unrooted phylogenetic tree. Caffeine metabolism, flavonoid, phenylpropanoid and terpenoids biosynthesis and xenobiotics degradation were typical in tea quality and drug therapy. We validated and enriched the genes by GO annotation and linked this to enzyme-powered pathways through interactive network map. A total of 93 KEGG pathways and associated genes encoded by more than 90% of the coding transcripts are responsible for the biosynthesis of these life-saving metabolites. Annotation of these transcripts revealed best hits for homology to discover more than 17,800 functional genes and conserved domains. To provide understanding of the naturally occurring tea and drug-specific products and the key pathways for the biosynthesis of these molecules, we sequenced the leaf transcriptome using Illumina MiSeq platform and generated 2,590,652 paired-end reads that were assembled de novo into 45,450 high-quality transcripts. In spite of this, there is no sequence information regarding this plant in literature. ![]() ex Harv) is one of the most valuable tea and traditional medicinal plants used in Southern Africa. ![]()
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